Chymotrypsin
CAS: 9004-07-3
Source: Bovine (Porcine) pancreas
General description
Chymotrypsin is also called chymotrypsinogen,which is the endopeptidase extracted and separatedfrom the fresh pancreas of cows or pigs.
Primarystructure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245amino acid residues, with 5 pairs of disulfide linkage in the molecule. Chymotrypsin is white and light yellow crystal or amorphouspowder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum operating PH is 8~9. It is stable at a dry state, but will be quicklydeactivated in water solution, most stable in the water solution with PH3~4.When it acts on the protein, it will hydrolyze the peptide bond formed by thecarboxyl of L-tyrosine and L-phenylalanine in priority.
The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd. ispurified by re-crystallization, and then by Chromatography andultra-filtration.
Specification
Chymotrypsin (USP)
Items | Specification | Method |
Appearance | White crystallized powder | USP33 |
Identification | No purple color develops within 3 minutes | USP33 |
Loss on drying | No more than 5.0% | USP33 |
Residue on Ignition | No more than 2.5% | USP33 |
Microbial limits | ||
Pseudomonas aeruginosa | Absent | USP33 |
Salmonella | Absent | USP33 |
Staphylococcus aureus | Absent | USP33 |
Limit trypsin | Not more than 1%. | USP33 |
Assay | NLT 1000 USP Chymotrypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency. | USP33 |
Storage
Should be sealed andstored in dark places, at temperature 2-8℃.
Chymotrypsin (EP)
Items | Specification | Method |
Description | white crystallize powder | EP6.3 |
Identification | A) A purple colour develops B)No colour develops with in 3 min of mixing | EP6.3 |
Appearance of solution | Solution S is not more opalescent than reference suspension Ⅱ | EP6.3 |
Absorbance | 1) 281nm : 18.5 ~ 22.5 2) 250nm : not more than 8.0 | EP6.3 |
PH | 3.0-5.0 | EP6.3 |
Histamine | Not more than 1µg/5.0µkatal | EP6.3 |
Loss on drying | Not more than 5% | EP6.3 |
Limit of trypsin | Not more than 1% | EP6.3 |
Assay | Not less than 5.0 ukatal/mg, calculated on the dried basis | EP6.3 |
Storage
Should be sealed andstored in dark places, at temperature 2-8℃.
Geyuantianrun is a China chymotrypsin manufacturer andsupplier. We offer chymotrypsin, chymotrypsinogen,and trypsin. Our high quality products are offered atcompetitive prices. Geyuantianrun is located in China, and the full chain of manufacturing trypsin-chymotrypsin, ribonuclease can be completed in China,even in one city. Lower manufacturing cost saves your purchasing cost. The moredetails of each product are shown on the page with description.