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Trypsin

Trypsin

CAS: 9002-07-7 
Source: Porcine pancreas or Bovine pancreas

Generaldescription
Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23,300 dalton, and is a singlepeptide chain composed of 223 amino acid residues. With rigorous specificity,the trypsin specifically acts on the peptide linkageconstituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation alsoreduces or loses gradually. It is easily soluble in the water, but insoluble intrichloromethane, ethanol, ether and glycerin, andthe optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated whenboiled for a short time; if the salt is added into the hot solution, theprotein will precipitate, and the enzyme action of filtrate cannot be seen, andCa2 plays the role in protecting and activating the trypsin.

The high purity trypsin of Beijing Geyuantianrun Bio-tech Co.,Ltd. is purified by re-crystallization, and then by ultra-filtration.

Specification  
1. Trypsin (USP)


Items

Specification

Method

Appearance

White crystallized powder

USP33

Solubility

500,000 USP Trypsin Unites, is soluble in 10mL of water and in 10 mL of saline TS

USP33

Loss on drying

≤5.0%

USP33

Residue on Ignition

≤2.5%

USP33

Microbial limits

Pseudomonas aeruginosa

Absent

USP33

Salmonella

Absent

USP33

Staphylococcus aureus

Absent

USP33

Limit chymotrypsin

NMT 50USP Chymotrypsin Units / 2500USP Trypsin Units (NMT approximately 5% of Chymotrypsin)

USP33

Assay

NLT 2500 USP Trypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency.

USP33



Storage

Trypsin should be sealed andstored in dark places, at temperature 2-8℃.

2. Trypsin (EP)


Items

Specification

Method

Description

White crystalline lyophilized powder

EP6.3

Identification

A) A purple color develops

B) No color develops with in 3 min of mixing

EP6.3

Appearance of solution

Solution S is not more opalescent than reference suspension Ⅲ

EP6.3

Absorbance

1) 280nm : 13.5 ~ 16.5

2) 250nm : not more than 7.0

EP6.3

PH

3.0-6.0

EP6.3

Histamine

Not more than 1µg/0.2µkatal

EP6.3

Loss on drying

Not more than 5%

EP6.3

Limit of trypsin

Not more than 1%

EP6.3

Microbial limits

Total viable aerobic count

Salmonella

E.Coli

≤10000cfu/g

Negative

Negative

EP6.3

Assay

Not less than 0.5 ukatal/mg, calculated on the dried basis

EP6.3



Storage
Should be sealed andstored in dark places, at temperature 2-8℃.

Packaging
1. 5kg aluminium containerpackaging (single-container)
Every 5kg product is contained in an aluminumcontainer, inside of which is lined with an aluminum foil bag to keep productsout of the light. The aluminum container is placed in a poly heat-insulated boxwhich is filled with cooling agent.

2. 5kg aluminium container packaging (dual-container)
Twocontainers are placed in one heat insulated box inwhich contains 10kg productstotally, and cooling agent is available in the heat insulated box.

3. 2.5kg aluminum container packaging
Every 2.5kg product is packaged in one container,inside of which is lined with an aluminum foil bag to keep products out of thelight. The aluminum container is placed in a poly heat-insulated box which isfilled with cooling agent.

4. 1kg aluminum container packaging
Every 1kg product is packaged in one container,inside of which is lined with an aluminum foil bag to keep products out of thelight. The aluminum container is placed in a poly heat-insulated box which isfilled with cooling agent.

5.Paperboard container packaging
Eachpaperboard container contains 5-10kg products, and inside of the container is lined with an aluminum foil bag tokeep products out of the light. The container is filled with cooling agent.

6. Aluminumfoil bag packaging
Suitable forsmall quantity product packaging for samples and small orders. The aluminiumfoil bag is placed inside a small heat-insulated box which is filled withcooling agent.

7.Centrifugal tube packaging
Suitable forsmall quantity product packaging for samples and small orders. The centrifugaltube is placed inside a small heat-insulated box which is filled with coolingagent.

8. Samplepackaging (heat insulated box)
Heatinsulated box is available for samples or small quantity products packaging,and it is filled with cooling agent.

9. Others
Productpackaging is also customizable.

Geyuantianrun is a major manufacturer of trypsin, basedin China.We offer various types of products such as trypsin, chymotrypsin, ribonuclease and soon. Our products are high quality and competitively priced. Our full chain ofmanufacturing chymotrypsinogen, trypsin-chymotrypsin can be completed in China,even in one city. Our lower manufacturing cost can save your purchasing cost. Amore detailed description of our product is shown on the page.


Trypsin


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