Trypsin
CAS: 9002-07-7
Source: Porcine pancreas or Bovine pancreas
Generaldescription
Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23,300 dalton, and is a singlepeptide chain composed of 223 amino acid residues. With rigorous specificity,the trypsin specifically acts on the peptide linkageconstituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation alsoreduces or loses gradually. It is easily soluble in the water, but insoluble intrichloromethane, ethanol, ether and glycerin, andthe optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated whenboiled for a short time; if the salt is added into the hot solution, theprotein will precipitate, and the enzyme action of filtrate cannot be seen, andCa2 plays the role in protecting and activating the trypsin.
The high purity trypsin of Beijing Geyuantianrun Bio-tech Co.,Ltd. is purified by re-crystallization, and then by ultra-filtration.
Specification
1. Trypsin (USP)
Items | Specification | Method |
Appearance | White crystallized powder | USP33 |
Solubility | 500,000 USP Trypsin Unites, is soluble in 10mL of water and in 10 mL of saline TS | USP33 |
Loss on drying | ≤5.0% | USP33 |
Residue on Ignition | ≤2.5% | USP33 |
Microbial limits | ||
Pseudomonas aeruginosa | Absent | USP33 |
Salmonella | Absent | USP33 |
Staphylococcus aureus | Absent | USP33 |
Limit chymotrypsin | NMT 50USP Chymotrypsin Units / 2500USP Trypsin Units (NMT approximately 5% of Chymotrypsin) | USP33 |
Assay | NLT 2500 USP Trypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency. | USP33 |
Storage
Trypsin should be sealed andstored in dark places, at temperature 2-8℃.
2. Trypsin (EP)
Items | Specification | Method |
Description | White crystalline lyophilized powder | EP6.3 |
Identification | A) A purple color develops B) No color develops with in 3 min of mixing | EP6.3 |
Appearance of solution | Solution S is not more opalescent than reference suspension Ⅲ | EP6.3 |
Absorbance | 1) 280nm : 13.5 ~ 16.5 2) 250nm : not more than 7.0 | EP6.3 |
PH | 3.0-6.0 | EP6.3 |
Histamine | Not more than 1µg/0.2µkatal | EP6.3 |
Loss on drying | Not more than 5% | EP6.3 |
Limit of trypsin | Not more than 1% | EP6.3 |
Microbial limits Total viable aerobic count Salmonella E.Coli | ≤10000cfu/g Negative Negative | EP6.3 |
Assay | Not less than 0.5 ukatal/mg, calculated on the dried basis | EP6.3 |
Storage
Should be sealed andstored in dark places, at temperature 2-8℃.
Packaging
1. 5kg aluminium containerpackaging (single-container)
Every 5kg product is contained in an aluminumcontainer, inside of which is lined with an aluminum foil bag to keep productsout of the light. The aluminum container is placed in a poly heat-insulated boxwhich is filled with cooling agent.
2. 5kg aluminium container packaging (dual-container)
Twocontainers are placed in one heat insulated box inwhich contains 10kg productstotally, and cooling agent is available in the heat insulated box.
3. 2.5kg aluminum container packaging
Every 2.5kg product is packaged in one container,inside of which is lined with an aluminum foil bag to keep products out of thelight. The aluminum container is placed in a poly heat-insulated box which isfilled with cooling agent.
4. 1kg aluminum container packaging
Every 1kg product is packaged in one container,inside of which is lined with an aluminum foil bag to keep products out of thelight. The aluminum container is placed in a poly heat-insulated box which isfilled with cooling agent.
5.Paperboard container packaging
Eachpaperboard container contains 5-10kg products, and inside of the container is lined with an aluminum foil bag tokeep products out of the light. The container is filled with cooling agent.
6. Aluminumfoil bag packaging
Suitable forsmall quantity product packaging for samples and small orders. The aluminiumfoil bag is placed inside a small heat-insulated box which is filled withcooling agent.
7.Centrifugal tube packaging
Suitable forsmall quantity product packaging for samples and small orders. The centrifugaltube is placed inside a small heat-insulated box which is filled with coolingagent.
8. Samplepackaging (heat insulated box)
Heatinsulated box is available for samples or small quantity products packaging,and it is filled with cooling agent.
9. Others
Productpackaging is also customizable.
Geyuantianrun is a major manufacturer of trypsin, basedin China.We offer various types of products such as trypsin, chymotrypsin, ribonuclease and soon. Our products are high quality and competitively priced. Our full chain ofmanufacturing chymotrypsinogen, trypsin-chymotrypsin can be completed in China,even in one city. Our lower manufacturing cost can save your purchasing cost. Amore detailed description of our product is shown on the page.