Automated On-line Sampling System

In the processes of cell cultivation and microbial fermentation, control of critical process parameters plays a vital role in ensuring successful experiment or production. Unlike conventional cultivation equipment that usually can only measure and control several common process parameters such as pH, DO, temperature, speed, etc., Jing Liang JL-YGZ600 automated on-line sampling system is capable of real-time controlling metabolites and calculating nutrient consumption in cell cultivation and microbial fermentation process, allowing user to precisely get information about the status of cell culture while achieving traceability.

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System Components

The automated on-line sampling system is mainly composed of three parts:
Automatic on-line sampling
Automatic biochemical analysis
Intelligent feedback control of fed-batch

Analysis Parameters of Sampling

Item	Range (not prediluted)	Range (prediluted)
Glucose	0.05~6g/L	0.05~60g/L
Lactic acid	0.05~5g/L	0.05~50g/L
Glutamate	15~1460mg/L	15~14600mg/L
Glutamine	15~1460mg/L	15~14600mg/L
Glycerin	0.05~2g/L	0.05~20g/L
Methanol	0.02~0.5g/L	0.01~10g/L
Ethanol (alcohol content)	0.02~1g/L	0.04~20g/L
Lysine	0.0~1.5g/L	0.0~15g/L
Xylose	0.05~2g/L	0.05~20g/L
Galactose	0.1~2g/L	0.1~20g/L
pH	6.0~8
Ammonium ion	0.01~0.6g/L	0.01~6g/L
Sodium ion	20.0~200mmol/L
Potassium ion	0.5~15mmol/L
Calcium ion	0.1~5mmol/L

Cases Fermentation Process Research and Development Project of Zhejiang SDSW Research Institute Project: Vitamin B2
Reaction: 96 hours
Item: glucose, lactic acid
Testing time: 10min
Object: 5L bioreactor
Result: workers’ day and night shifts are cancelled during the cell culture process, feeding accuracy and product expression rate are improved by high-frequency sampling testing and feedback controlling of fed-batch. Cervical Cancer Vaccine Project of Jiangsu RKSW Pharmaceutical Technology Co., Ltd. Project: vaccine for cervical cancer
Reaction: 80 hours
Item: glycerin
Testing time: 20min
Object: three 30L bioreactors
Result: workers’ day and night shifts are cancelled during the cell culture process, feeding accuracy and product expression rate are improved by high-frequency sampling testing and feedback controlling of fed-batch. YGF300 biochemistry analyzer Performance Parameters: Sampling frequency and sampling rate can be set according to actual needs
Fully automatic aseptic operation in sampling process
Sampling rate: 2-10 ml/min
Sampling interval: Min. 5min
Sample volume: 0.5-5 ml